报告题目：Co-regulation of multiple genes promote inner ear progenitors to regenerate hair cells in the mouse cochlea

报告人：柴人杰 教授

时  间：2020年9月24日 下午2:30

地  点：徐州医科大学主校区科教楼D403

主持人：肖诚

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Abstract ：Sensory hair cells in the inner ear are responsible for sound recognition. Damage to hair cells in adult mammals causes permanent hearing impairment because these cells cannot regenerate. By contrast, newborn mammals possess limited regenerative capacity because of the active participation of various signaling pathways. The Wnt, Notch and Atoh1 are highly sophisticated and conserved signaling pathways that control multiple cellular events necessary for the formation of sensory hair cells. All three signaling pathways allow resident supporting cells to regenerate hair cells in the neonatal cochlea. Foxg1 is one of the forkhead genes that are involved in morphogenesis, cell fate determination, and proliferation, and Foxg1 was previously reported to be required for morphogenesis of the mammalian inner ear. We found that Foxg1 conditional knock-down (cKD) in Sox2+ SCs and Lgr5+ progenitors at postnatal day (P)1 both led to large numbers of extra inner HCs in P7 mouse cochleae, and these extra inner HCs survived at least to P30 and had normal hair bundles and synapses. Pou4f3 and Gfi1, are orderly expressed in the process of HC differentiation and maturation. The Pou4f3 gene is an essential gene for HC formation in the cochlear development, and also is a target of ATOH1 in HCs. Gfi1 is a target of the Pou4f3 gene, and is essential for the survival of cochlear HC. Considering the possible roles of these genes in mammalian hair cell regeneration and maturation, we co-regulated these genes simultaneously in postnatal cochlear supporting cells, and explored the roles of these genes in SC-to-HC conversion and maturation of new HCs.

主讲人简介：柴人杰，东南大学生命科学与技术学院教授(2013年至今)，博士生导师，副院长。2004年本科毕业于中国科学技术大学生物学专业，导师施蕴渝院士。2009年博士毕业于美国Baylor大学，生物医学专业。2009-2013年于Stanford大学医学院从事博士后工作。2013年8月开始在东南大学生命科学研究院任教授。一直从事神经元和内耳毛细胞再生和保护的研究，以及神经干细胞和内耳干细胞转录调控机制的研究。近5年(2015-2020)发表SCI论文87篇（总影响因子602.9）；其中第一和通讯作者论文64篇（总影响因子385.5，其中IF>20分2篇，IF 10-20分8篇, IF 8-10分4篇），期刊包括Cell, Nature communications, Science Advances, PNAS，Autophagy, Advanced Materials, Advanced Science, Advanced Functional Materials，Biomaterials等。获中组部青年千人、国家自然基金委优秀青年、江苏省杰出青年、江苏双创团队、江苏双创人才、江苏333工程人才等称号。主持科技部国家重点研发计划干细胞及转化研究重点专项和青年973计划，中国科学院“器官重建与制造”A类战略性先导科技专项，军委科技委军事脑科学项目，国家自然科学基金委面上项目，15项横向课题。在中国生物物理学会、中国生理学会、中国细胞生物学会、中国神经科学会等下属分会任理事、会长、主委或副秘书长等。